A group of mucin type glycoprotein Sialosyl Lewis Antigens (SLA), such as CA19-9 and CA19-5, have come to be recognized as circulating cancer associated antigens for gastrointestinal cancer. CA19-9 represents the most important and basic carbohydrate tumor marker. The immunohistological distribution of CA19-9 in tissues is consistent with the quantitative determination of higher CA19-9 concentrations in cancer than in normal or inflamed tissues. Recent reports indicate that the serum CA19-9 level is frequently elevated in the serum of subjects with various gastrointestinal malignancies, such as pancreatic, colorectal, gastric and hepatic carcinomas. Together with CEA, elevated CA19-9 is suggestive of gallbladder neoplasm in the setting of inflammatory gallbladder disease. This tumor-associated antigen may also be elevated in some non-malignant conditions. Research studies demonstrate that serum CA19-9 values may have utility in monitoring subjects with the above-mentioned diagnosed malignancies. It has been shown that a persistent elevation in serum CA19-9 value following treatment may be indicative of occult metastatic and/or residual disease. A persistently rising serum CA19-9 value may be associated with progressive malignant disease and poor therapeutic response. A declining CA19-9 value may be indicative of a favourable prognosis and good response to treatment.

CA 19-9 ELISA: 

Immunoenzymatic colorimetric method for quantitative determination of CA19-9 in human serum or plasma.

Principle of the Assay:

Microtiter strip wells are precoated with monoclonal antibodies against CA19-9. During first incubation CA19-9 in samples and standards bind to the immobilised antibodies on the surface of the microtiter wells. Unbound substances will be removed by a subsequent washing step. During second incubation specific anti-IgG antibodies conjugated with peroxidase bind to CA19-9. Unbound conjugate will be removed by a subsequent washing step. In a third incubation the enzyme substrate TMB will be oxidized resulting in a blue colour. Addition of sulphuric acid (stop solution) stops the enzymatic reaction and turns the blue reaction product into yellow. Absorbance is measured at 450 nm. The amount of oxidized TMB is proportional to the amount of CA19-9 in the sample.

Specific performance characteristics:

Order information: