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Total Estriol
Estriol is a female sex steroid hormone and one of the three major naturally occurring estrogens, the others being estradiol and estrone. Estriol is produced almost exclusively during pregnancy and is the major estrogen produced in the normal human fetus. During pregnancy the production of Estriol depends on an intact maternal-placental-fetal unit. Fetal-placental production of Estriol leads to a progressive rise in maternal circulating levels reaching a late-gestational peak several orders of magnitude greater than non- pregnant levels. In non-pregnant circumstances, Estriol is derived almost exclusively from 17β-Estradiol. Estriol exists in biological matrix in free (ca. 9 %) and conjugated form (91 %). Conjugation with glucuronic or sulphuric acid of estriol occurs in the liver, which aides in steroid solubility, therefore allowing rapid elimination via the kidney.
May be diagnosed by:
- Determination of specific antibodies based on the ELISA technique
- Determination of specific antibodies based on the RIA-technique
Total Estriol ELISA:
The Total Estriol ELISA is intended for competitive immunoenzymatic colorimetric method for quantitative determination of Estriol in human serum or plasma.
Principle of the Assay:
Microtiter strip wells are precoated with anti-Estriol antibodies (solid-phase). Estriol in the sample competes with added horseradish peroxidase labelled Estriol (enzyme-labelled antigen) for antibody binding. After incubation a bound/free separation is performed by solid-phase washing. The immune complex formed by enzyme-labelled antigen is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product. The intensity of this product is inversely proportional to the amount of Estriol in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorption at 450 nm is read using an ELISA microwell plate reader.
Specific performance characteristics:
Intraassay | Interassay | Sensitivity | Accuracy | |
CV% | CV% | ng/ml | ±SE | |
Total Estriol | 6.1 | 3.6 | 1 | 94% ± 4.0% |
Correlation with another commercially available free testosterone assay performed on 32 samples is r = 0.987
Order information:
ELISA | Number of Determinations | Product Number |
Total Estriol | 96 | DNOV011 |
