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Anti-TG

Thyroglobulin (TG) is a protein produced by and used entirely within the thyroid gland. Thyroglobulin is used by the thyroid gland to produce the thyroid hormones thyroxine (T4) and triiodothyronine (T3). Thyroglobulin synthesis is stimulated at the transcriptional level by thyroid-stimulating hormone (TSH).

Thyroglobulin (TG) is a well-known target for autoantibodies occurring in thyroid autoimmunity (Graves' disease and Hashimoto's thyroiditis). Anti-TG antibodies mostly belong to the IgG class. Low to moderate levels of anti-TG antibodies can be found in sera of other autoimmune patients (eg systemic lupus erythematosus or Sjogren syndrome). In some cases anti-TG positive sera may show negativity for other type of anti-thyroid antibodies - anti-TPO. Therefore, combined determination of both types of anti-thyroid antibodies (anti-TPO + anti-TG) provides most sensitive laboratory diagnostic tool for thyroid autoimmunity. Separately from autoimmunity, anti-TG antibodies may develop in patients suffering from thyroid cancer. High level of anti-TG in such patients may interfere with correct determination of serum thyroglobulin, which serves as tumour marker for therapy control in this group of patients.

Anti-TG ELISA:

Immunoenzymatic colorimetric method (ELISA) for quantitative determination of Anti-TG in serum or plasma.

Principle of the Assay:  

This test is based on two-site sandwich enzyme immunoassay principle. The mircoplates are coated with Streptavidin. In the first step the antigen Thyroglobulin which is conjugated to Biotin binds to Streptavidin. Then the sample is placed into the microwells now coated by the antigen. Antibodies from the sample bind coated antigen on the microwell surface. Unbound material is removed by a washing procedure. Second antibodies directed against human IgG and labelled with horseradish peroxidase, are then added to the microwells. A second washing step is performed. Then the immune complex is visualized by adding Tetramethylbenzidine (TMB) substrate, which gives a blue reaction product. The intensity of this product is proportional to the amount of Anti-TG in samples and standards. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorption at 450 nm is read using an ELISA microwell plate reader.

Specific performance characteristics: 

Intraassay

Interassay

Analytic Sensitivity

Accuracy

CV%

CV%

IU/ml

±SE

Anti-TG

4.2

6.4

5.0

98.5% ± 4.6%

Correlation with commercially available anti-TG assay performed on 54 samples is r = 0.99

Order information:

ELISA

Number of Determinations

Product Number

Anti-TG

96

DNOV115

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Anti-TG

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Anti-TG

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