+4 9 (0) 60 7 4 487 6 0
standing the test of time

Bordetella pertussis and Bordetella pertussis toxin (PT)

Bordetella pertussis is the causative organism for whooping cough, which affects only humans. In general, Bordetella are small, coccoid Gram-negative rods (see figure). The pathogens are transmitted by aerosol droplets. Sources of infection are infected persons during the catarrhal phase, who cough out. The bacteria are able to attach themselves to the cells of the ciliated epithelium in the bronchi. They rarely invade the epithelium. The infection results in (sub-) epithelial inflammations and necroses. The onset of whooping cough develops after an incubation period of 1-3 weeks in three phases (see the table below).

Pertussis occurs worldwide. In Africa for example Bordetella pertussis is - besides the measles virus - mainly responsible for the high lethality during the first years of life. Especially children, who visit the kinder garden, give currency to the pathogens also patients with sub clinical experience. Beside it adults, who vaccinated as children, have little or no residual immunity and fall sick.

Species

Disease

Symptoms

Mechanism of infection

Bordetella pertussis

Whooping cough(Pertussis)

Catarrhal phase: lasting 1-2 weeks, uncharacteristic. Paroxysmal phase: lasting 2-3 weeks, with typical convulsive coughing spells.

Decrementi phase: convalescent phase, which can last for several weeks.

Transmission by aerosol droplets with high risk of infection.

Humans are the only hosts.

Incubation period: 1-3 weeks.

Infections may be diagnosed by:

  • Microscopy: determination in cultures, IF
  • Serology: determination of specific antibodies based on the ELISA-technique

A precise diagnostic is necessary for an effective therapy, the isolation of non-vaccinated children and differentiation of whooping cough and atypical diseases resp. chronic infections.

NovaLisaTM Bordetella pertussis IgA/IgG/IgM ELISA:

NovaLisa® Bordetella pertussis IgA/IgG/IgM ELISA is intended for the qualitative determination IgA-, IgG- and IgM- class antibodies against Bordetella pertussis in human serum or plasma (citrate).

Antigens:

IgA, IgG: B. pertussis Toxin and filamentous Hemagglutinine. IgM Purified lysate antigens of B. pertussis

Specific performance characteristics:

Intraassay

Interassay

Sensitivity

Specificity

n

Mean

CV%

n

Mean

CV%

IgA

20

0.47

7.1

12

1.28

5.18

87.5%

>90%

24

0.88

4.9

12

2.40

6.94

IgG

20

0.55

3.9

12

15

4.9

97.6%

94%

24

1.48

5.2

12

35

6.1

IgM

20

0.44

8.5

12

0.85

9.4

84.2%

>95%

24

1.04

14.0

12

2.25

8.2

 

NovaLisa® Bordetella pertussis toxin (PT) IgA/IgG ELISA: (NEW)

The NovaLisa® Bordetella pertussis toxin (PT) IgA/IgG ELISA is intended for the quantitative determination of IgA/IgG-class antibodies to Bordetella pertussis toxin (PT) in human serum or plasma (citrate).

Antigens: B. pertussis toxin (PT)

Recommended Interpretation according to current Publications*

IgG anti-PT

IgA anti-PT

<40 IU/ml

not indicative of recent contact

<12 IU/ml

not indicative of recent contact


≥40 - <100 IU/ml

testing of a second sample after 7-10 days or further analysis of the antibody response is recommended

<12 IU/ml

not indicative of recent contact

≥12 IU/ml

indicative of recent contact

≥100 IU/ml

indicative of recent contact

≥12 IU/ml

indicative of recent contact

* Riffelmann, M.; Thiel, K.; Schmetz, J.; Wirsing von Koenig, C.H. (2010) Performance of commercial enzyme-linked immunosorbent assays for detection of antibodies to Bordetella pertussis. J Clin Microbiol. Dec;48(12):4459-4463

Specific performance characteristics:

Intraassay

Interassay

Sensitivity

Specificity

Analytical Sensitivity

n

Mean (IU/ml)

CV%

n

Mean (E)

CV%

IgG

12

129.0

6.9

24

2.030

2.2

> 95%

>95%

< 5 IU/ml

12

12.8

9.9

23

0.659

3.7

IgA

12

5.2

7.3

23

0.547

8.0

> 98%

88.9%

< 3 IU/ml

12

16.3

4.4

24

1.954

2.0

12

6.2

8.2

24

1.048

2.7

 

Order information:

ELISA

Number of determinations

Product Number

Bordetella pertussis IgA

96

BOPA0030

Bordetella pertussis IgG

96

BOPG0030

Bordetella pertussis IgM

96

BOPM0030

Bordetella pertussis toxin (PT) IgG

96

BPTG0610

Bordetella pertussis toxin (PT) IgA

96

BPTA0610