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Avidity Toxoplasma gondii 2nd Generation

Toxoplasma gondii is a small intracellular parasite, whose live cycle has a sexual and an asexual phase. Sexual development is restricted to the intestinal cells of (probably exclusively) cats; the oocysts formed are excreted and due to their resistant cell walls they may be infectious under advantageous circumstances for at least 1 year. Animals and man are intermediate hosts for the asexual proliferation of T. gondii: the ingested parasites will proliferate explosively within the host cells lysing them eventually. They disseminate throughout the body via circulation and lymphatic system and though may infect any cell type. In muscle and brain cells cysts are formed which are spheroidal and about 5-100 μm in diameter. Cysts are virtually immortal in the intermediate hos

Toxoplasma gondii is the most common parasite in humans, but its abundance (7-80 %) is highly dependent on the geographic area, the socioeconomic status and the nutritional customs. Infection only rarely causes toxoplasmosis and usually clinical symptoms are absent, but may produce severe problems in immunosuppressed persons and fetus.

Because only a primary infection during pregnancy may be dangerous and even fatal for the unborn (the probability of congenital infection is about 50 %), the recent onset of an infection must be excluded.

In pregnant women in over 98 % of cases, the absence of IgM excludes the possibility of recent infection. In newborns the very presence of anti-toxoplasma IgM is sufficient to confirm a congenital toxoplasmosis, since maternal IgM, unlike IgG, does not cross the placental barrier. But a significant number of infected infants do not develop detectable IgM levels and thus are false negative. In immunosuppressed patients toxoplasmosis causes severe complications mostly by reactivation of an earlier latent infection.

 

Species

Disease

Symptoms

Mechanism of Infection

Toxoplasma Godndii

Toxoplasmosis

Acquired Toxopasmosis:
lymphadenopathy, chorioretinitis

Congenital Toxoplasmosis:
hydrocephalus, microcephaly, intracranial calcifications, chronical chorioretinitis

Ingestion of oocysts by food, including water contaminated feces of cats or contaminated soil

Ingestion of cysts by eating raw or insufficiently cooked meat, esp. pork

Congenital infection


Infections may be diagnosed by:  

  • PCR (detection of viral RNA)
  • EIA, ELISA (detection of specific antibodies).

The presence of IgG antibodies to Toxoplasma gondii indicates the occurrence of the infection but does not distinguish between recent and past infection. Virus-specific IgM antibodies are first detected ten days and peak at about four weeks post infection. They may persist for more than seven months after acute infections. Based on the evidence that antibody avidity gradually increases after exposure to an immunogen, avidity of IgG antibodies can be used as a marker for distinguishing recent primary from long-term infections. Avidity describes the binding strength of a specific antibody to its antigen. Low-avidity IgG antibodies indicate a primary infection, whereas the presence of IgG antibodies with high avidity points to persistency or reactivation of infection.

Intend Use

The NovaLisa® Avidity Toxoplasma gondii IgG ELISA is intended to indicate the T. gondii-specific IgG avidity in human serum or plasma (citrate, heparin) to differentiate between acute and past infection


Performance Characteristics:

The NovaLisa® Avidity Toxoplasma gondii IgG Test has been evaluated for use in Toxoplasmosis with samples of acute and past infections. A total number of 84 defined patient samples were tested. These samples were supplied by the Institute of Medical Microbiology, Immunology and  Parasitology, University Bonn.  





Defined Samples

Low Avidity

High Avidity

Total

Agreement
(%)

NovaLisa® Avidity Toxoplasma gondii IgG Test

Low Avidity

46

0

46

100

High Avidity

0

38

38

100

Total

46

38

84

100


Order information:

ELISA

Number of Determinations

Product Number

Toxoplasma IgG

96

TOXG0460

Toxoplasma IgM µ-capture

96

TOXM0460

Toxoplasma IgG Avidity Test

96

ATOX7460

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info(at)novatec-id.com

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Performance Characteristics

Avidity Toxoplasma IgG