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Chikungunya

The Chikungunya Virus is a single-stranded, enveloped RNA-Virus that belongs to the genera Alphavirus of the togavirus family (Togaviridae). The entire virion measures 60-65 nm. 

Relating to the geographic occurrence the virus is divided into five variations: the West-African, the East- and South-African, the one of the Indian Ocean and the Asiatic. 

Chikungunya-Viruses are transmitted to humans by bloodsucking mosquitoes
(Aedes aegypti, Aedes albopictus). In general the viruses are not transmitted from human to human but transmissions from infected pregnant women to unborn children have been proved. 

The symptoms of Chikungunya include fever which can reach 39°C (102,2°F) a petechial or maculopapular rash usually involving the limbs and trunk, and arthralgia or arthritis affecting multiple joints which can be debilitating.

The symptoms can also include headache, conjunctival injection and slight photophobia. The fever typically lasts for two days and abruptly comes down. However other symptoms, namely joint pain, intense headache, insomnia and an extreme degree of prostration last for a variable period, usually for about five to seven days. But patients have complained joint pain for much longer time period depending on age of the patient. It has been observed that the severity of the disease as well as its duration is less in younger patients and pregnant women. Heavy damages to somebody?s health or death are rare.

Species

Disease

Symptoms

Mechanism of Infection

Chikungunya

Chikungunya Fever

Fever which can reach 39° (102.2°F)

Maculopapular rash

Arthritis

Transmission to humans by mosquito bites from Aedes aegypti as well as Aedes albopictus mosquitoes

Infections may be diagnosed by:

  • Microscopy: Determination of Alphaviruses in cell cultures during the acute phase.  
  • PT - PCR Serology: Determination of specific antibodies based on the ELISA technique

NovaLisa? Chikungunya IgG/IgM µ-capture ELISA:

The NovaLisa? Chikungunya IgG/IgM µ-capture ELISA is intended for the qualitative determination of IgG and IgM class antibodies against Chikungunya virus in human serum.

Antigens:

Chikungunya antigens
 
Specific performance characteristics:


Intraassay

Interassay

Sensitivity %

Spezificity %


n

Mean

CV%

n

Mean

CV%



IgG

24

1.44

2.7

3(68)

1.41

3.9

>90

>90

IgM

23

0.62

4.8

3(69)

0.61

7.3

>90

>90

 Order information:

ELISA

Number of Determinations

Product Number

Chikungunya IgG

96

CHIG0590

Chikungunya IgM µ-capture

96

CHIM0590

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Product Insert

Chikungunya IgG