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Dengue

Dengue fever, also known as breakbone fever, is an infectious tropical disease caused by the dengue virus and transmitted by mosquitoes. Dengue fever virus (DENV) is a virus of the family Flaviviridae, genus Flavivirus and contains a single-stranded RNA genome with positive polarity. There are four serotypes of the virus, which are referred to as DENV-1, DENV-2, DENV-3 and DENV-4.

The geographical distribution is around the equator, particularly Latin America, Central Africa, India, Southeast Asia, Western Pacific and South of the USA.

Dengue viruses are transmitted to humans through the bites of infective female yellow fever mosquitoes (Stegomyia aegypti, formerly Aedes aegypti). The mosquitoes generally acquire the virus while feeding on the blood of an infected person. After virus incubation for eight to ten days, an infected mosquito is capable, during probing and blood feeding, of transmitting the virus for the rest of its life.

Yellow fever mosquitoes are well adapted to living in close proximity to humans, and to feeding off people rather than other vertebrates. They prefer to lay their eggs in artificial water containers, such as flower vases, uncovered barrels, buckets and discarded tires.

The incubation period ranges from 3-14 days, but most often it is 4-7 days. Typically, people infected with dengue virus are asymptomatic or only have symptoms of a common cold. The characteristic symptoms of dengue are sudden-onset fever (up to 40 °C) with intense headache (especially behind the eyes), and muscle and joint pain. In combination with a skin rash this symptoms are known as the "dengue triad". This usually lasts 3-7 days. In some patients the disease proceeds to a critical phase.

Dengue Hemorrhagic Fever (DHF) or Dengue Shock Syndrome (DSS) occur in less than 5 % of all cases of dengue. About 1-5 % of severe cases are fatal. In individual epidemics the case-fatality rate may reach up to 15 %.

Infection with one serotype is believed to produce lifelong immunity to that serotype but only short term protection against the others. Secondary infection with a different serotype may result in severe clinical manifestations. There is no vaccination available.

Species

Disease

Symptoms

Mechanism of Infection

Dengue Virus

Dengue Fever

After an incubation phase of 1-2 weeks ague, fever, headache, muscle pain and in some cases exanthema occur. After 7 days the illness is over

Transmission by mosquitoes (Stegomyia aegypti)

Dengue hemorrhagic fever (DHF)
or Dengue shock syndrome (DSS)

With a high lethality rate involving hemorrhages and intravasal coagulation.
Weak rapid pulse, narrow pulse pressure

Infections may be diagnosed by:

  • Virus Detection: Determination of the isolated virus, RT-PCR, NS1 antigen detection
  • Serology: Detection of specific antibodies by ELISA, immunofluorescence (IF), neutralization test (NT).

NovaLisaTM Dengue IgG/IgM/IgM µ-capture ELISA:

The NovaLisaTM Dengue IgG/IgM/IgM µ-capture ELISA is intended for the qualitative determination IgG-/IgM-class antibodies against Dengue viruses in human serum or plasma (citrate).

Antigens:

Purified Dengue Virus 2 antigens from strain 16681.

Specific performance characteristics:

Intraassay

Interassay

Sensitivity

Specificity

n

Mean

CV%

n

Mean

CV%

IgG

 8

0.98

4.3

8

0.47

6.8

>90%

93%

IgM

 7

0.97

3.1

20

1.18

4.88

90%

97.6%

IgM µ-capture

23
24
24

0.530
1.019
0.986

3.2
2.4
2.8

12
12
12

20.0
9.0
5.3

4.8
6.2
5.8

>98%

97.5%

 

Order information:

ELISA

Number of Determinations

Product Number

Dengue IgG

96

DENG0120

Dengue IgM

96

DENM0120

Dengue Virus IgM µcapture

96

DVM0640

 

 

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Dengue

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Dengue IgG