Disclaimer
Product Inserts on this website are made available for ilustration purposes only. When using one of our test kits, one must use the insert provided with the kit itself.
CA 15-3
CA 15-3, is an abbreviation for cancer antigen 15-3.
CA 15-3 levels are most useful in following the course of treatment in women diagnosed with breast cancer, especially advanced breast cancer.
CA 15-3 levels are rarely elevated in women with early stage breast cancer. Cancers of the ovary, lung, and prostate may also raise CA 15-3 levels. Elevated levels of CA 15-3 may be associated with noncancerous conditions, such as benign breast or ovarian disease, endometriosis, pelvic inflammatory disease, and hepatitis. Pregnancy and lactation can also cause CA 15-3 levels to rise.
CA15-3 is most useful for monitoring patients post-operatively for recurrence, particularly metastatic diseases. 96% of patients with local and systemic recurrence have elevated CA15-3, which can be used to predict recurrence earlier than radiological and clinical criteria. A 25% increase in the serum CA15-3 is associated with progression of carcinoma. A 50% decrease in serum CA15-3 is associated with response to treatment. CA15-3 is more sensitive than CEA in early detection of breast cancer recurrence. In combination with CA-125, CA15-3 has been shown to be useful in early detection of relapse of ovarian cancer. CA15-3 levels are also increased in colon, lung and hepatic tumours.
CA15-3 ELISA:
Immunoenzymatic colorimetric method for quantitative determination of CA15-3 in human serum.
Principle of the Assay:
Microtiter strip wells are precoated with monoclonal antibodies against CA15-3. During first incubation CA15-3 in samples and standards bind to the immobilised antibodies on the surface of the microtiter wells. Unbound substances will be removed by a subsequent washing step. During second incubation specific antibodies conjugated with peroxidase bind to CA15-3. Unbound conjugate will be removed by a subsequent washing step. In a third incubation the enzyme substrate TMB will be oxidized resulting in a blue colour. Addition of sulphuric acid (stop solution) stops the enzymatic reaction and turns the blue reaction product into yellow. Absorbance is measured at 450 nm. The amount of oxidized TMB is proportional to the amount of CA15-3 in the sample.
Specific performance characteristics:
Intraassay | Interassay | Analytical Sensitvity | |
| CV% | CV% | U/ml |
CA15-3 | 5.9 | 7.7 | 5 |
Order information:
ELISA | Number of Determinations | Product Number |
CA15-3 | 96 | DNOV062 |
